By Louis Klein
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D. e. D. values. This can be prevented either by flash pasteurization, or by acidification to pH 3-0 followed by neutralization; the sample must then be seeded with fresh settled sewage (free from nitrifying bacteria) to supply the necessary organisms (see Chapter 11, page 125). D. of river waters containing algae can be misleadingly high if the test is carried out, as is usually the case, in the dark. The algae will exert an extra oxygen demand on account of respiration and possibly also their organic content.
A useful routine method of estimating sulphides in river waters has been developed by H O U L I H A N a n d F A R I N A 2 5 1 , a n d is based u p o n the application of the Winkler technique used for the determination of dissolved oxygen. ), followed by the alkaline iodide azide reagent, a n d after settling the precipitate, N / 8 0 iodine* is added a n d t h e mixture is acidified a n d then titrated with N / 8 0 sodium thiosulphate. Absorption of iodine is d u e to sulphides a n d any other substances which react with iodine.
151 BRIGGS and his co-workers have described a continuous recording apparatus for dissolved oxygen based on the Winkle? method which is adapted to automatic working by determining the liberated iodine photometrically. A modification of the Winkler titration method was used by TRUESDALE and his associates152 in a recent re-determination of the solubility of oxygen in water when exposed to air. m. of oxygen. Another titration method for dissolved oxygen, finding occasional application in field and control tests, is that due to MILLER 1 6 » 154» 155 and depends upon the oxidation of ferrous salts at a high pH value to ferric salts, tartrate being present to prevent any precipitation of ferric hydroxide.