Diagnostic Biosensor Polymers by Arthur M. Usmani, Naim Akmal

By Arthur M. Usmani, Naim Akmal

content material: Diagnostic polymers and coatings : chemistry, know-how, and purposes / Arthur M. Usmani --
Fiber-optic sensors in response to degradable polymers / Venetka Agayn and David R. Walt --
Enhancement of the steadiness of stressed out quinoprotein glucose dehydrogenase electrode / Ling Ye, Ioanis Katakis, Wolfgang Schuhmann, Hanns-Ludwig Schmidt, Johannis A. Duine, and Adam Heller --
Amperometric glucose-sensing electrodes with using converted enzymes / F. Mizutani, S. Yabuki, and T. Katsura --
Electron-transport premiums in an enzyme electrode for glucose / Nigel A. Surridge, Eric R. Diebold, Julie Chang, and Gerold W. Neudeck --
Sandwich-type amperometric enzyme electrodes for choice of glucose / S. Mutlu, M. Mutlu, P. Vadgama, and E. Pişkin --
Reproducible electrodeposition approach for immobilizing glucose oxidase and a differentially permeable outer-membrane fabric to be used on a miniature implantable glucose sensor / K.W. Johnson, D.J. Allen, J.J. Mastrototaro, R.J. Morff, and R.S. Nevin --
Thin-layer flow-through enzyme immunosensor according to polycaprolactam web / Lizhi Yin, Keli Xing, Hong Du, Hui Miao, Xi Wang, and Yuanming Li --
carrying out polymers and their software in amperometric biosensors / Wolfgang Schuhmann --
Poly(ether amine quinone)s as electron-transfer relay platforms in amperometric glucose sensors / Hsing Lin Lan, T. Kaku, Hiroko I. Karan, and Yoshiyuki Okamoto --
Flow-injection research of a few anionic species : automatic, miniaturized, twin operating electrode potentiostat utilizing a poly(3-methylthiophene)-modified electrode / Elmo A. Blubaugh, George Russell, Merril Racke, Dwight Blubaugh, T.H. Ridgway, and H.B. Mark, Jr. --
Characterization of balance of transformed poly(vinyl chloride) membranes for microfabricated ion-selective electrode arrays in biomedical functions / Ernö Lindner, Vasile V. Cosofret, Tal M. Nahir, and Richard P. dollar --
Biosensors according to ultrathin movie composite membranes / Charles R. Martin, Barbara Ballarin, Charles Brumlik, and Del R. Lawson --
Viologen spinoff containing polysiloxane as an electron-transfer mediator in amperometric glucose sensors / Hiroko I. Karan, Hsing Lin Lan, and Yoshiyuki Okamoto --
Hydrogen peroxide electrodes in response to electric connection of redox facilities of assorted peroxidases to electrodes via a three-d electron-relaying polymer community / Mark S. Vreeke and Adam Heller --
New biocompatible polymer : program for implantable glucose sensor / Kazuhiko Ishihara, Nobuo Nakabayashi, Kenro Nishida, Michiharu Sakakida, and Motoaki Shichiri --
Biocompatibility of perfluorosulfonic acid polymer membranes for biosensor functions / R.F.B. Turner and C.S. Sherwood --
Nondegradable and biodegradable polymeric debris : practise and a few chosen biomedical purposes / E. Pişkin, A. Tuncel, A. Denizli, E.B. Denkbaş, H. Ayhan, H. Çiçek, and K.T. Xu --
Semisynthetic macromolecular conjugates for biomimetic sensors / Mizuo Maeda, Koji Nakano, and Makoto Takagi --
Enzyme immobilization on polymerizable phospholipid assemblies / Alok Singh, Michael A. Markowitz, Li-I Tsao, and Jeffrey Deschamps --
managed unencumber from liposomes of long-chain polymerizable diacetylenic phosphocholine and a short-chain saturated phospholipid / Michael A. Markowitz, Eddie L. Chang, and Alok Singh --
Immobilization of glucose oxidase on polyethylene movie utilizing a plasma-induced graft copolymerization strategy / Chee-Chan Wang and Ging-Ho Hsiue --
Spatially managed on-wafer and on-chip enzyme immobilization utilizing photochemical and electrochemical innovations / David J. Strike, Albert van den Berg, Nico F. de Rooij, and Milena Koudelka-Hep --
electric communique among glucose oxidase and electrodes according to poly(vinylimidazole) advanced of bis(2,2'-bipyridine)-N, N'-dichloroosmium / Ravi Rajagopalan, Timothy J. Ohara, and Adam Heller.

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These characteristics are particularly promising for the use of the electrode in flow injection measuring systems. Therefore we examined the analytical potential of the modified GOD/Nafion-coated GC electrode by measuring glucose in beverages. Figure 3 shows typical responses by the electrode. The peak current was proportional to the glucose concentration up to 10 m M , under the present exrjerimental conditions, and the detection limit was 10 μΜ. Table I gives the results for the determination of glucose in beverages.

GCE I exhibited a GOD activity of ca. 2 U cm~2, whereas GCE II exhibited an activity lower than 1 m U cm"2. In the case of CPE II, the native GOD placed on the electrode surface easily leaked through the Nafion overcoat, and only a small amount of the enzyme remained immobilized. In contrast, the water-insoluble, lipid- modified GOD was stable between the electrode surface and the polymer overcoat which permitted the much higher enzyme activity. Glucose Response of Enzyme Electrode. Figure 2 shows current-time curves for GCE I and II.

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